Owi Taq Hot Start Master Mix is a ready-to-use 2X PCR mix containing Owi Taq Hot Start DNA polymerase, dNTPs, MgCl2, and an optimised ammonium-based buffer. The enzyme is chemically modified: a group temporarily blocks its active site, rendering it inactive at room temperature. This inhibition allows for safe reaction preparation without the risk of non-specific amplification. Enzyme activity is restored during the initial denaturation step at 94 to 95°C, ensuring true Hot Start PCR with high specificity, fewer primer dimers, and improved yields, even on complex matrices. The Ready-to-Load version also contains a migration dye and loading buffer, allowing for direct loading onto a gel after PCR. Simply add your primers, matrix, and water to get started.
- Ready to load: includes loading dye for direct gel analysis
- High yields and high specificity
- Highly efficient on GC/AT contents
- Direct-colony PCR: no DNA extraction required
- Robust amplification: up to 5kb
- Master mix format with MgCl2 included
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